Literature summary for 2.7.7.B22 extracted from

Sato, S.; Arimura, Y.; Kujirai, T.; Harada, A.; Maehara, K.; Nogami, J.; Ohkawa, Y.; Kurumizaka, H.
Biochemical analysis of nucleosome targeting by Tn5 transposase (2019), Open biology, 9, 190116 .

Search for more literature for 2.7.7.B22

Protein Variants

Protein Variants	Comment	Organism
E54K/L372P	mutations lead to a hyperactive Tn5 transposase	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q46731	-	-

Synonyms

Synonyms	Comment	Organism
Tn5	-	Escherichia coli
TnpA	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	Tn5 transposase preferentially targets near the entry-exit DNA regions within the nucleosome. Tn5 transposase minimally cleaves the dinucleosome without a linker DNA. A linker DNA length of 10-15 base-pairs is important for the efficient Tn5 integration reaction. In the presence of a 30 base-pair linker DNA, Tn5 transposase targets the middle of the linker DNA, in addition to the entry-exit sites of the nucleosome	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)